Journal: Clinical and Experimental Medicine

Article Title: The association between 4-HPR-mediated LCN2 suppression and reduced intestinal cell senescence in ulcerative colitis

doi: 10.1007/s10238-025-01843-4

Figure Lengend Snippet: LCN2 was highly expressed in UC. A P16 was upregulated in Caco-2 cells after the treatment of LPS (n = 3, p -value < 0.0001). P21 was upregulated in Caco-2 cells after the treatment of LPS (n = 3, p -value = 0.0451), LCN2 was upregulated in Caco-2 cells after the treatment of LPS (n = 3, p -value = 0.0005). B The differentially expressed LCN2 between the normal and UC tissues (Symbols and lines, n = 4, p -value = 0.0036). C The expression levels of P16 and P21 following treatment with the LCN2 inhibitor or 4-HPR (n = 3). D – E Colon tissue images and corresponding quantified colon lengths after various treatments (n = 5). F The 7-day body weight change curve (n = 5). G Time-dependent changes in DAI scores following treatment (n = 5). The data of WB analysis was presented as Symbols and lines, the other data are presented as mean ± SEM, * < 0.05, ** < 0.01, *** < 0.001, **** < 0.0001

Article Snippet: To delineate the mechanistic pathway, P21 and P16 expression levels were assessed after LPS stimulation in the presence or absence of either the LCN2 inhibitor ZINC00640089 (MedChemExpress) or 4-HPR (MedChemExpress). qRT-PCR was also employed to verify the overexpression of LCN2 in CD4 + cells.

Techniques: Expressing

Journal: Clinical and Experimental Medicine

Article Title: The association between 4-HPR-mediated LCN2 suppression and reduced intestinal cell senescence in ulcerative colitis

doi: 10.1007/s10238-025-01843-4

Figure Lengend Snippet: 4-HPR alleviated colitis-associated clinical symptoms and enhanced the integrity of the intestinal mucosal barrier in mouse model. A Study design for 4-HPR treatment in mice model with DSS-induced acute colitis. B – C The figure and analysis of colon length of mice (n = 6). D Body weight changes of mice during the experimental cycle (n = 6). E Disease activity index (DAI) score (n = 6). F – G HE staining analysis of intestinal tissues of three groups (n = 5), scale bar: 100 μm. H – I Serum FITC-dextran 4 KD concentration and lipopolysaccharide (LPS) concentration of three groups (n = 5). J Representative images of colonic ZO-1 and Occludin measured by immunofluorescence, scale bar: 40 μm. Data are presented as mean ± SEM, * < 0.05, ** < 0.01, *** < 0.001, **** < 0.0001

Article Snippet: To delineate the mechanistic pathway, P21 and P16 expression levels were assessed after LPS stimulation in the presence or absence of either the LCN2 inhibitor ZINC00640089 (MedChemExpress) or 4-HPR (MedChemExpress). qRT-PCR was also employed to verify the overexpression of LCN2 in CD4 + cells.

Techniques: Activity Assay, Staining, Concentration Assay, Immunofluorescence

Journal: Clinical and Experimental Medicine

Article Title: The association between 4-HPR-mediated LCN2 suppression and reduced intestinal cell senescence in ulcerative colitis

doi: 10.1007/s10238-025-01843-4

Figure Lengend Snippet: 4-HPR attenuated intestinal tissue aging in UC by inhibiting LCN2. A The expression level of senescence-associated genes P16, P21, and LCN2 (n = 5). B The SA-β-gal staining and analysis in colon tissues of three groups (n = 5), scale bar: 200 μm. C Safety assessment of 4-HPR as a single-agent treatment (n = 5). Data are presented as mean ± SEM, **** < 0.0001; ns, no significance

Article Snippet: To delineate the mechanistic pathway, P21 and P16 expression levels were assessed after LPS stimulation in the presence or absence of either the LCN2 inhibitor ZINC00640089 (MedChemExpress) or 4-HPR (MedChemExpress). qRT-PCR was also employed to verify the overexpression of LCN2 in CD4 + cells.

Techniques: Expressing, Staining

Journal: Clinical and Experimental Medicine

Article Title: The association between 4-HPR-mediated LCN2 suppression and reduced intestinal cell senescence in ulcerative colitis

doi: 10.1007/s10238-025-01843-4

Figure Lengend Snippet: 4-HPR regulated the Th17/Treg balance by LCN2. A The levels of inflammatory cytokines of three groups (n = 3). B The gating strategy in flow cytometry. C The distribution of Th17/Treg by flow cytometry analysis (n = 3). D The overexpression of LCN2 was confirmed by qPCR (n = 4). E The secretion level of IL-10 and IL-17A (n = 4). Data are presented as mean ± SEM, * < 0.05, ** < 0.01, *** < 0.001, **** < 0.0001

Article Snippet: To delineate the mechanistic pathway, P21 and P16 expression levels were assessed after LPS stimulation in the presence or absence of either the LCN2 inhibitor ZINC00640089 (MedChemExpress) or 4-HPR (MedChemExpress). qRT-PCR was also employed to verify the overexpression of LCN2 in CD4 + cells.

Techniques: Flow Cytometry, Over Expression